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| Silva Júnior, Jessé Marques da; Universidade Federal de Lavras - UFLA; Paiva, Renato; Universidade Federal de Lavras - UFLA; Campos, Ana Carolina Atala Lombelo; Universidade Federal de Lavras - UFLA; Rodrigues, Marcelo; Universidade Federal de Lavras - UFLA; Carvalho, Milene Alves de Figueiredo; Universidade Federal de Lavras - UFLA; Otoni, Wagner Campos; Universidade Federal de Viçosa - UFV. |
| The technique of hybridization using plant protoplasts is widely used in plant breeding programs. The purpose of our study is to further characterize the process of protoplast isolation from the ornamental species Etlingera elatior (Jack) R. M. Smith. Protoplasts were isolated from different tissues: in vitro leaves, in vitro pseudostem, and leaves from plants cultivated hydroponically. We tested six enzymatic combinations, four incubation time periods, the rotary system (40 rpm) or steady in the dark, and three concentrations of mannitol (0.5, 0.6 and 0.7 M). The diameter and viability of obtained protoplasts were evaluated. The best source of explants used for protoplast isolation was the in vitro leaves, which yielded 22x105 protoplasts g-1 of fresh... |
| Tipo: Pesquisa Empírica |
Palavras-chave: Fisiologia Vegetal; Biotecnologia Vegetal; Melhoramento Genético FDA; Ornamental plant; Enzymatic combinations; Incubation period Melhoramento Vegetal. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12309 |
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| Silva, Marcus Vinícius Vieira da; Instituto Federal Goiano; Sales, Juliana de Fátima; Instituto Federal Goiano; Silva, Fabiano Guimarães; Instituto Federal Goiano; Neto, Aurélio Rubio; Instituto Federal Goiano; Alberto, Paula Sperotto; Instituto Federal Goiano; Pereira, Flávia Dionísio; IFGoiano - Campus Rio Verde. |
| The objective of this study was to evaluate the physiological quality of babassu (Orbignya phalerata Mart.) embryos as a function of desiccation. The fruits were placed in a convection oven at 37±2°C for 0, 3, 6, 9, or 12 days. After these periods, the moisture contents of the fruits and seeds were determined. A reduction in the water content occurred more rapidly in the fruits than in the seeds. The decrease in seed moisture content from 12.59% (on the basis of fresh weight) to 4.56% did not affect the percentage of germinated zygotic embryos; however, we did observe a decline in the vigor as the seed moisture content decreased. Embryos with a 4.56% moisture content developed into seedlings of shorter lengths after 30, 60, and 90 days of in vitro growth.... |
| Tipo: Pesquisa Empírica |
Palavras-chave: 5.01.03.00-8 Arecaceae; Desiccation tolerance; Tissue culture; Fitotecnia. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13429 |
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